Vitamin D Hydroxy (25-OH) Testing and Deficiency

25-hydroxy vitamin D blood test is done to check the vitamin D level in our body.

This form of vitamin is converted into a more active form by the kidney. This active form is directly responsible for controlling calcium and phosphorus levels in the blood.

The normal range for vitamin D 25-OH in blood is 30.0 to 74.0 nanograms per milliliter (ng/ml).

Symptoms Suggesting Vitamin D Deficiency

  • Muscle pains in one or more muscle groups of the body. Pain may be in one muscle at some time and another muscle at some other time.
  • Joint pain
  • Weight gain
  • Headaches
  • Constipation or diarrhea
  • Poor concentration
  • Restlessness, during sleep
  • Fatigue in general

Causes of Low Vitamin D Levels

Vitamin D deficiency is very common these days. There can be many causes for this. They are:

  • Dietary deficiency of the vitamin
  • Lack of exposure to sunlight
  • Poor food absorption
  • Liver or kidney diseases
  • Certain medicines like, phenytoin, some barbiturates etc.

Medical Advice (Q&As) on “Hydroxy Vitamin D 25-OH Blood Test Levels and Deficiency Symptoms

  1. Npra

    Age 8 years female. Often complaints about pain in toes (left) feels good while pressing the toes. What could be the reason? How to get rid of this pain?

    1. Buddy M.D. Post author

      Is she having her daily glasses full of milk? This could be vitamin D deficiency. You may get her blood levels checked up and go for treatment.

      A daily supplement of calcium with Vitamin D may be started.


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